AI Modelling and Time-series Forecasting Systems for Trading Energy Flexibility in Distribution Grids

We demonstrate progress on the deployment of two sets of technologies to support distribution grid operators integrating high shares of renewable energy sources, based on a market for trading local energy flexibilities. An artificial-intelligence (AI) grid modelling tool, based on probabilistic graphs, predicts congestions and estimates the amount and location of energy flexibility required to avoid such events. A scalable time-series forecasting system delivers large numbers of short-term predictions of distributed energy demand and generation. We discuss the deployment of the technologies at three trial demonstration sites across Europe, in the context of a research project carried out in a consortium with energy utilities, technology providers and research institutions

Origin and quantification of circulating DNA in mice with human colorectal cancer xenografts

Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an experimental system to systematically examine these aspects. Nude mice were xenografted with human HT29 or SW620 colorectal carcinoma (CRC) cells and ctDNA was analyzed by Q–PCR with highly specific and sensitive primer sets at different times post-graft. We could discriminate ctDNA from normal (murine) cells and from mutated and non-mutated tumor (human) cells by using species-specific KRAS or PSAT1 primers and by assessing the presence of the BRAF V600E mutation. The concentration of human (mutated and non-mutated) ctDNA increased significantly with tumor growth. Conversely, and differently from previous studies, low, constant level of mouse ctDNA was observed, thus facilitating the study of mutated and non-mutated tumor derived ctDNA. Finally, analysis of ctDNA fragmentation confirmed the predominance of low-size fragments among tumor ctDNA from mice with bigger tumors. Higher ctDNA fragmentation was also observed in plasma samples from three metastatic CRC patients in comparison to healthy individuals. Our data confirm the predominance of mononucleosome-derived fragments in plasma from xenografted animals and, as a consequence, of apoptosis as a source of ctDNA, in particular for tumor-derived ctDNA. Altogether, our results suggest that ctDNA features vary during CRC tumor development and our experimental system might be a useful tool to follow such variations

Speech Communication Antecedents of Perceived Confirmation

Two studies sought to examine the relationship between facilitative communication, self‐disclosure, and agreement/disagreement of one person in a marital relationship and the other\u27s feeling of being confirmed. Differences in communication behaviors associated with male feelings of being confirmed and female feelings of being confirmed were discovered. Further, agreement and disagreement were not found to contribute to feelings of being confirmed. Findings are discussed in relation to the theoretical structure of confirming/disconfirming communication